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Index one: the i7 index column is inside the forward orientation (match the orientation Should the i7 indexes from the sample sheet).
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If operating in manual manner and working with bcl2fastq or BCL Change, or uploading the sample sheet to BaseSpace for a manual manner operate and attaching a sample sheet, enter the i5 sequence inside the forward path.
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Therefore these computer software Focus on the assumption that the i5 sequence is usually from the ahead orientation and will routinely build the reverse enhance of the sequences supplied for the appropriate instrument sort.
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